Development of an Agarose Gel Electrophoresis Technique for Determining a-Amylase lsoenzymes

Glass slide-based agarose gel electrophoresis for determining the efficiency of RNA amplification and cyanine dye incorporation during RNA labeling.

The use of electroimmunoassay for determining specific proteins as a supplement to agarose gel electrophoresis.

Electrophoretic transfer as a technique for the detection and identification of plant amylolytic enzymes in polyacrylamide gels.

An electrophoretic transfer technique was developed for the specific identification of isozymes of starch debranching enzyme, alpha-amylase, and beta-amylase. Amylolytic enzymes are separated by native polyacrylamide slab gel electrophoresis and proteins in gels are electrophoretically transferred through starch-containing polyacrylamide gels. Each amylolytic enzyme degrades starch in the trans...

Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathies.

Capillary zone electrophoresis (CZE) and immuno-subtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples were analyzed with CZE and AGE to determine sensitivity and specificity in detecting IFE-confirmed mon...

Evaluation of light-scattering index (nephelometry) for assessing serum triglycerides and lipoprotein phenotypes.

Light-scattering measurements (nephelometry) of serum after filtration through appropriate filters is a rapid method for physically determining triglyceride concentrations and lipoprotein phenotypes. A correlation coefficient of greater than 0.9 was obtained when aliquots of the same specimen were tested for triglycerides by light scattering in an Aminco-Bowman Spectrophotofluorometer and by a ...